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Fourty three kinds of Cymbidium including C. kanran, C. latifolium, C. hosai var. hakuran, C. gyokuchin, C. formosanum, C. forrestii and C. georingii were self- or cross-pollinated and characteristics of resulting seeds were investigated. Also the effects of growth regulators and kinds of media on rhizome proliferation and organogenesis were studied. The seeds matured on the average of 307 days and germinated on 277 days. Variations in maturation and germination were greatly depending on species and success of fertilization, and various abnormalities such as no or double embryo from one seed were observed. The seed size varied greatly, and their length was 1.0∼1.7㎜ and width was 0.15∼0.25㎜. Seed germination was obtained with Knudson medium supplemented with 10% coconut milk. Hyponex media was effective in organogenesis and growth than Knudson or Murashige-Skoog media, but effectiveness varied with kind of media, species, success of fertilization and types of auxin. Substantial rhizome growth was obtained with low kinetin/NAA, while good shoot formation and growth occurred with high ratio. The ratio of kinetin 3.0㎎/ℓ/NAA 0.3㎎/ℓ induced in vitro flowering from rhizome in C. niveo-marginatum and C. ensifolium x C. kanran (Japan) `Dogui´.
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